Polydopamine-based biofunctional substrate coating promotes mesenchymal stem cell migration

Abstract Rapid migration of mesenchymal stem cells (MSCs) on device surfaces could support in vivo tissue integration and might facilitate vitro organoid formation. Here, polydopamine (PDA) is explored as a biofunctional coating to effectively promote MSC motility. It hypothesized that PDA stimulates fibronectin deposition this way enhances integrin-mediated capability. The random directional cell was investigated by time-lapse microscopy gap closure assay respectively, analysed with softwares computational tools. A higher amount deposited observed substrate, compared the non-coated substrate. integrin ?1 activation focal adhesion kinase (FAK) phosphorylation at Y397 were enhanced but F-actin cytoskeleton not altered, suggesting regulated initiated FAK signalling. This study strengthens biofunctionality for regulating offering facilitating devices. Graphic abstract Polydopamine-coated substrate induces increased cells, promotes via integrin-initiated signaling, polystyrene-based standard culture surface. In way, multifunctional implant surface